| The Bulletín of Kanagawa Dental College. | |
| Vol. 35 No. 2 September 2007 | |
| ISSN: 0385-1443 UBIC: 65 | |
| Abstract | |
| Porphyromonas gingivalis plays an important role in the initiation and progression
of periodontal disease. In the present study, we tested whether the biochemical inhibition of tryptophanase,
with the competitive inhibitor oxindolyl-L-alanine, could affect polystyrene colonization by Porphyromonas gingivalis.
Oxindolyl-L-alanine inhibits, in a dose-dependent manner, indole production by strain ATCC 33277 grown in
BHI-broth. Oxindolyl-L-alanine aiso inhibits the adherence ofATCC 33277 cells to polystyrene. At 0.5 mg/ml,
oxindolyl-L-alanine exhibits inhibitory activity against biofilm formation in BHI-broth. These data suggest
that índole, produced by the action of tryptophanase, is involved in polystyrene colonization by indole-producing
bacteria, P. gingivalis. Indole may act as a signalling molecule to regulate the expression of adhesion and
biofilm-promoting factors. Key words: Oxindolyl-L-alanine, Porphyromonas gingivalis, Biofílm, indole. |
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